| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 981 | Thermobifida fusca | AGTGGATGCTGCGCAACT | TAGACCTCGACGAGCAGCA | 59.97 | 60.38 | 193 | 82 |
100.00%
|
100.00%
|
| Primer 982 | Thermobifida fusca | GTCCGCACCATCATGGAGT | ACGCAGGTGAAAGACGCA | 59.78 | 59.89 | 228 | 82 |
100.00%
|
100.00%
|
| Primer 983 | Thermobifida fusca | CAGTCTGCGCAAACTGCA | AGGAAGTGGTTCTCGCTGC | 58.98 | 60.00 | 258 | 82 |
100.00%
|
100.00%
|
| Primer 984 | Thermobifida fusca | AATGAACGCTCCGGTGCA | TTTGAGCAGCAGACCCACC | 59.97 | 60.23 | 175 | 82 |
100.00%
|
100.00%
|
| Primer 985 | Thermobifida fusca | ACGCGAAACCGTGGAACT | AGTTCACCGTCAGCGACAG | 59.89 | 60.01 | 164 | 82 |
100.00%
|
100.00%
|
| Primer 986 | Thermobifida fusca | ACGCGAAACCGTGGAACT | TCACCGTCAGCGACAGTTC | 59.89 | 60.01 | 161 | 82 |
100.00%
|
100.00%
|
| Primer 987 | Thermobifida fusca | ACGCGAAACCGTGGAACT | TCAGTTCACCGTCAGCGAC | 59.89 | 60.01 | 166 | 82 |
100.00%
|
100.00%
|
| Primer 988 | Thermobifida fusca | TGGGAGTACGACGTCGACT | CGCTGGGGTCGTCTTTGAT | 60.00 | 60.08 | 286 | 82 |
100.00%
|
100.00%
|
| Primer 989 | Thermobifida fusca | TCAACACACTGCTCGGCA | AGTAGGCGATCACCAGTGC | 59.50 | 59.48 | 174 | 82 |
100.00%
|
100.00%
|
| Primer 990 | Thermobifida fusca | AAGGTTCGTCTGACCGCA | AACTCGAGGAGCCGTTCAC | 58.87 | 59.71 | 176 | 82 |
100.00%
|
100.00%
|